Journal: bioRxiv

Article Title: A human synovial tendon-on-a-chip models key features of peritendinous adhesions and offers a new approach methodology for testing anti-fibrotic drugs

doi: 10.64898/2026.04.03.716316

Figure Lengend Snippet: a , Immunofluorescent confocal images of Ki67 (yellow) in the synovial gel (SG) adjacent to the tendon gel (T) at Days 1 and 5 without exogenous TGF-β1. Quantification of Ki67+ cells and total synovial cell density demonstrate elevated proliferation at Day 1 and an increased overall cell density in the synovial gel by Day 5. b, Immunofluorescent confocal images and quantification of CD90 (green) expression with Hoechst (blue) as nuclear counterstain at Days 1 and 5 demonstrate enrichment of reparative CD90+ cells in the synovial gel. c, Immunofluorescent confocal images and quantification of podoplanin (PDPN, yellow) expression within the synovial gel, demonstrating significant upregulation from Day 1 to Day 5. d, Three-dimensional confocal imaging of live CellTracker-labeled monocytes (red) at Days 1, 3, and 5 showing transendothelial migration into the (i) synovial and (ii) tendon compartments (±FLS). Quantification of monocyte infiltration, reported as the number of transmigrated monocytes mm -3 , shows time-dependent increase in monocyte infiltration in +FLS compared to -FLS devices at Days 3 and 5. Data are mean ± s.d.(n=4 devices per condition); Two-way ANOVA with Tukey’s post-hoc test; *p<0.05, ***p<0.001.

Article Snippet: Immunofluorescence images of collagen III, Ki67, and CD90 were analyzed in Imaris.

Techniques: Expressing, Imaging, Labeling, Migration

Journal: bioRxiv

Article Title: A human synovial tendon-on-a-chip models key features of peritendinous adhesions and offers a new approach methodology for testing anti-fibrotic drugs

doi: 10.64898/2026.04.03.716316

Figure Lengend Snippet: a , Representative confocal images of Ki67 (yellow) and F-actin (red) in the synovial gel (SG) adjacent to the tendon construct (T) at Days 1 and 5 in -FLS devices without exogenous TGF-β1. Quantification of Ki67+ cells within the synovial gel and total synovial cell density, reported as cells mm -3 . b, Representative confocal images of CD90 (green) and Hoechst (blue) in the synovial gel at Days 1 and 5 (-FLS). Quantification of CD90⁺ cells within the synovial gel, demonstrating increased CD90⁺ fibroblast density at Day 5. Data are mean ± s.d. (n = 4 devices per condition); unpaired t-test; *p<0.05, **p<0.01.

Article Snippet: Immunofluorescence images of collagen III, Ki67, and CD90 were analyzed in Imaris.

Techniques: Construct